Watching this, which I do fairly often, is always a joy.
So many memorable bits, but my current favorite begins with the menacing look Bud gives Curly, immediately followed by the breathy/confusing 'hi-ho' from Kitty, and ends with one simple musical note as Curly is shaving. That has always been one of my favorite sound effects.
For non guitar players, next time you come in contact with an acoustic, pluck the thickest string (on top). You will laugh, and no one else will understand why low E is so hilarious.