That's in Cactus Makes Perfect, released in 1942.
One of the funniest, most "cartoony" Stooges shorts, and one of my all-time favorites. I love what happens right after Curly bids his fond farewell to Betsy the Cow, and the Stooges go off to seek their fortune. Everything we've seen so far has led us to believe that they live on a farm somewhere way out in the country, but they walk across the street and it turns out that Mother Stooge's farmhouse is right downtown in the middle of a big city!
A little touch of comic genius, there... I wonder how many people notice it the first time they see it? It took me a couple of viewings to catch it.